AWWA PATH56521

Identification and Characterization of Infectious and Non-infectious Sub-Populations of Encephalitizoon intestinalis Spores Purified from in vitro Cell Culture

American Water Works Association , 09/22/2002

Publisher: AWWA

File Format: PDF

$12.00$24.00

Description

Standard By:American Water Works Association , 09/22/2002

Published:09/22/2002

Pages:5

File Size:1 file , 120 KB

Language:English

Note:This product is restricted and cannot be purchased in the following countries Ukraine, Russia, Belarus

This report describes flow cytometric identification, cell and molecular characterization of two E. intestinalis subpopulations isolated from cell culture propagated, Percoll-purified spore suspensions. The results of the molecular analysis of both spore subpopulations indicated that both populations possessed E. intestinalis 16S rRNA and ¿-tubulin genes. However, only one subpopulation was able to infect RK-13 cells. Researchers should be aware that there is inherent variability in the viability of microsporidia spores propagated using cell culture. Includes 11 references.

AWWA PATH56521

Identification and Characterization of Infectious and Non-infectious Sub-Populations of Encephalitizoon intestinalis Spores Purified from in vitro Cell Culture

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