AWWA WQTC65727

The objective of this study was to evaluate, in parallel, endocrine disruptions of both estrogenic and thyroid hormone peripheral functions generated by complex mixtures of organic micropollutants. Water samples were collected in two major wastewater treatment plants (WWTPs) of the greater Paris area. Treatment processes are different where one is based on classical activated sludge steps (600 000 m³/day) and the other on biofilters (240 000 m(sup>3/day). Samples were taken at the inlet and outlet of the WWTPs and in the river Seine downstream of the discharge and at the intake of drinking treatment plants producing water for the Paris suburb. Chemicals were isolated from collected waters by SPE extraction (Waters - OASIS cartridges) and were tested using two cellular models for estrogenic (MELN) and thyroid disruption evaluations. Prior to the assessment of the estrogenic and THs activities, the cytotoxicity of the compounds for the cells was investigated using the MTT assay (Mosmann, 1983). Includes 6 references.