BS PD ISO/TS 19006:2016

Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

BSI Group , 08/31/2016

Publisher: BS

File Format: PDF

$132.00$264.16

Description

Standard By:BSI Group , 08/31/2016

Published:08/31/2016

Pages:32

ISBN(s):9780580832246

File Size:1 file , 3.1 MB

Language:English

Note:This product is unavailable in Ukraine, Russia, Belarus

BS PD ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation inRAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomeratesusing the CM-H2DCFDA assay.The protocol in this document is limited to use of a 24 well plate so if other plates were to be used,volumes would need to be adjusted and the protocol steps validated to ensure confidence in the testresults.Cross References:ISO 10993-5:2009ISO 29701:2010ISO/TS 80004-2:2015ISO/TS 18827ISO/TR 13097:2013

BS PD ISO/TS 19006:2016

Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

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