BS PD ISO/TS 21569-5:2016

BS PD ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified(GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 basepairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GMplants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may besuitable also for analysis of other products such as feedstuffs and seeds. The procedure requires theextraction of an adequate quantity and quality of amplifiable DNA from the test sample.The DNA sequence amplified by the P-FMV element-specific method can be detected in samples whichcontain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirma positive screening result. Further analyses are required using construct-specific or event specificmethods.Cross References:ISO 21569ISO 21570ISO 21571:2005ISO 24276ISO/IEC 17025ISO 16577